A monocentric study on the assessment of the safety culture at the Institute for Cardiovascular Diseases aimed to investigate the level of the safety culture of the patients themselves as assessed by the employees of the institute, to determine whether there are differences in aspects with regard to the place of work among the respondents, whether there is a connection with the variables that are closely related to the workplace, and examine whether the frequency of reporting unwanted events is also reported. The research was conducted at the Dubrava Clinical Hospital at the Institute for Heart and Blood Vessel Diseases, and the total number of respondents was 46. The respondents were nursing professionals of the institute itself. The survey is completely anonymous and was conducted live. It consists of eight sections in which various segments of the researched are examined. The time needed to carry out the research was from April to May 2023. The results showed that all respondents are in direct contact with patients on a daily basis, the majority of respondents work in medical non-surgical departments, the largest number of respondents have been employed at the Dubrava Clinical Hospital for more than 21 years. The examined data indicate that the majority of respondents in the last 12 months did not fill out and report an unwanted event, and the reason given was lack of support from management staff, fear of condemnation and/or disciplinary measures. By encouraging communication and teamwork, we promote the improvement of the safety culture process and consequently minimize the occurrence of unwanted events. Putting patient safety in the focus of all health and non-health workers is the primary goal in order to bring the level of health care provided to the highest possible level.