Human papillomaviruses (HPV) are DNA viruses that infect the basal epithelial cells of the skin and mucous membranes. There are low-risk and high-risk HPV genotypes that can lead to cervical cancer. The immune system of most infected individuals is able to eliminate the virus, but if it remains present, it can lead to development of cancer. HPV infections are classified as benign, premalignant, and malignant, depending on the risk of cancer development. HPV is transmitted through direct or indirect contact and can also be passed from mother to child during pregnancy. Diagnosis is made through clinical, histological, and cytological examinations, as well as molecular biology methods. Although there is no specific treatment for HPV infection, vaccination is an effective preventive measure. Prevention is based on vaccination, screening programs, and education. Nurses and technicians play a key role in the prevention and early detection of cervical cancer through education and support for at-risk groups. This research, conducted from June to August 2024 at the University North in Varaždin, analyzed differences in the level of awareness, habits, and attitudes towards HPV among nursing and physiotherapy students. The study included 108 respondents, of whom 68 were in nursing studies and 40 in physiotherapy studies. The results revealed certain differences, indicating the need for a tailored educational approach. One of the key findings of the research is the insufficient level of knowledge about HPV in both groups. Although nursing students more frequently correctly identified the link between HPV and cervical cancer, general knowledge about transmission methods and the importance of vaccination remains limited, especially among physiotherapy students. This highlights the need for additional education on prevention, transmission, and vaccination against HPV. The role of highly educated nurses is particularly important in addressing this issue. As future healthcare professionals, nurses play a key role in educating and raising awareness among patients about the importance of HPV prevention and regular medical check-ups. Therefore, it is crucial for both nursing and physiotherapy students to acquire adequate knowledge and skills so that they can effectively educate and advise their future patients, thereby contributing to reducing the incidence of HPV infections and related diseases.