People are the bearers of the quality of every organization, including healthcare institutions. The enormous importance of human resources in this industry requires a planned, systematic and thoughtful management of human resources. People are the bearers of the quality of every organization, including healthcare This paper deals with the management of human resources in a healthcare institution, and the aim of the paper is to analyze the theoretical aspects of human resources management in healthcare, as well as to analyze the state of Croatian healthcare in terms of human resources. Also, the goal of the paper is to gain insight into how human resources management is implemented in the institutions where the respondents are employed, and to evaluate the expediency and quality of such systems through practical research conducted by interviewing healthcare workers. Given that twelve respondents participated in the research, the results of the research should not be generalized. However, the results undoubtedly point to certain problems and challenges faced by the management of human resources in healthcare institutions, which ultimately reflects on the entire healthcare system, i.e. its functionality and quality. In order to overcome challenges and reduce problems, suggestions for improvement were made.