Healthcare associated infections affect a large part of the population. They represent a major threat to patient safety and a financial problem for healthcare. One of the most important measures to prevent healthcare associated infections is a hand hygiene. It includes hygienic washing of hands with liquid soap and water and hygienic rubbing of alcohol-based hand rub. There are specific signs that indicate the moments when hand hygiene is performed, and they are contained in the concept of "5 moments for hand hygiene". The biggest problem with hand hygiene is thought to be the low level of adherence to hand hygiene guidelines by healthcare workers. From January to March 2023, a cross-sectional survey was conducted among nurses and technicians employed in healthcare institutions. The research was conducted through an online survey, and the aim was to examine the knowledge, attitudes and practice of nurses and technicians about the hygienic habits of hand washing in healthcare institutions. The KAP survey consisted of 4 parts; sociodemographic data of respondents, knowledge about hygienic hand washing habits, attitudes and implementation of hand hygiene. A total of 115 respondents, between the ages of 18 and 55 and older, took part in the research. A higher percentage of respondents was female (87,8%) and having completed undergraduate studies in nursing (37,4%). From the answers it can be seen that the participating nurses and technicians have positive attitudes regarding hand hygiene in a higher percentage. The majority of respondents (83.5%) prefer to wash their hands with liquid soap and water rather than use alcohol-based disinfectants. About a third of respondents (33.9%) stated that they always rub an alcohol-based disinfectant on their hands after washing their hands with liquid soap and water. Continuous education is essential for nurses and technicians in the prevention of diseases related to the provision of health care by influencing their knowledge, attitudes and practices regarding hand hygiene.