OBJECTIVE: The aim of this paper was to examine the attitudes and knowledge of parents in Varaždin County about human papillomavirus vaccination. We also aimed to examine parents' beliefs about vaccination itself, and what causes them concern about immunization. RESPONDENTS AND METHODS: The research was conducted in the period from August 1, 2020. until 31.08.2024. The survey questionnaire was conducted through the internal Google docs platform. For the purposes of this research, a questionnaire called "Knowledge and attitudes of parents of Varaždin County about HPV vaccination" was used as a measuring instrument, which was created for the purpose of conducting this research. The first part of the questionnaire examined the sociodemographic data of the respondents, while the main part of the questionnaire focused on questions about parents' knowledge and attitudes about vaccination. Data were processed with descriptive statistics, presenting continuous data as mean values and standard deviations, and categorical data as absolute and relative frequencies. 400 respondents from Varaždin County participated in the research. RESULTS: A total of 400 respondents participated in the research, of which 343 (85.5%) were female. With regard to the age of the respondents, the largest share of respondents is 41 - 45 99 (24.6%) and 36 - 40 41 (22.6%) years old. With regard to the place of residence, 286 (71.5%) respondents indicate a rural environment. According to the level of education, the largest share of respondents mentioned secondary school 248 (61.7%). Out of the total number of respondents, 325 (80.8%) stated that they did not have a medical profession. The largest number of respondents, 147 of them (36.6%) stated that they learned that there is a vaccine against human papillomavirus from a doctor specializing in school medicine. Answers that examined parents' knowledge about the human papilloma virus show that 360 (89.6%) respondents know what HPV is, 340 (84.6%) respondents know whether HPV causes cervical cancer and 339 (84. 3%) of respondents. CONCLUSION: Considering all the results of the research, we can see that there is statistical significance in the attitudes and knowledge of parents about vaccination against HPV with regard to the age of the respondents, the level of education and the place of residence. HPV is the most common sexually transmitted infection in the world, and that is why it is important to get vaccinated against HPV at an early age, and to educate young people and parents about its importance.