Microorganisms are the simplest forms of life, and their development started more than three billion years ago, which means that we can view them as the most adaptable organisms that inhabit every part of the earth, but also every part of the human body. Their ability to adapt to adverse conditions and unpredictable behavior are one of the hallmarks of their successful survival. In recent years, the term MRSA – methicillin-resistant Staphylococcus aureus – has been increasingly mentioned in medical terminology. This term refers to a bacterial agent that has developed specific resistance to antistaphylococcal drugs that are a member of the group of beta-lactam antibiotics. The significance of this bacterial species is that treatment is more difficult than in other diseases, and the organism may be present in inpatient and outpatient settings. The research on the knowledge and attitudes of nursing professionals on MRSA has been conducted on a total sample of 245 respondents from Croatia in the period between 15th and 25th of July, 2021 in an online format. Two thirds of the respondents belonged to the age group up to 40 years of age, while a total of 91% of the respondents were female. The majority of respondents (96%) answered correctly that MRSA is actually methicillin-resistant Staphylococcus aureus. A total of 92% of respondents believe that contact isolation is pursued in patients infected with MRSA. Furthermore, 86% of respondents believe that the most common route of transmission of MRSA is through the hands of health care staff who have been in contact with an infected or colonized patient, and that optimal hand hygiene is performed at the patient care site (77%).