Healthcare-associated infections are a major public health problem. Given that special attention has recently been paid to patient's safety, prevention and control of the occurrence of infections associated with treatment and health care are one of the most important components of patient's safety care. During hospitalization, patients are exposed to numerous pathogens in the environment from various sources such as healthcare staff or other patients. For this reason, it is necessary to apply adequate measures to prevent the spread of hospital infections in highrisk patients. Most often, these are contact isolation measures, whose aim is to reduce and prevent the transmission of infection from one patient to another or from health workers to patients. At the same time, contact isolation measures are a fundamental measure in preventing infections with multiresistant organisms that appeare more and more often. As a basic level of infection control, standard protective measures are used, as a part of the routine approach to every patient and are recommended for working with all patients, regardless of their infectious status. The task of nurses is to know the measures of standard protection, risk factors for hospital infections and the spread of multiresistant organisms, the implementation of contact isolation measures in cases where there is a need for one, and continuous education related to the same. For the purposes of the work was conducted the reasearch. The aim of it was to examine the knowledge and opinion of nurses/technicians about contact isolation measures in relation to the sociodemographic characteristics of the research participants (age, level of education and work experience), additional education, and whether they implement contact isolation measures at their workplace. A total of 144 nurses/technicians from the Republic of Croatia participated in the research. The questionnaire was distributed via a Google docs document in several Facebook and Viber groups whose members are the nurses/technicians themselves. The research was conducted in the period from April 25, 2022 to July 15, 2022. The conducted questionnaire consisted of a total of three parts. In the first part, respondents were asked to provide sociodemographic data and whether they were familiar with contact isolation measures and the existence of protocols and guidelines at their workplaces. The second and third parts of the questionnaire contained questions/assertions related to contact isolation measures, and these parts were taken as a measure of knowledge of nurses/technicians about contact isolation measures of patients. A total of 3 hypotheses were set during the creation of the survey questionnaire. One of the set hypotheses was partially confirmed, while the other two were not confirmed.