The COVID-19 pandemic has affected millions of people around the world since December 2019. For the purpose of disease prevention, a number of measures were introduced that greatly affected the lives of all residents so far. From self-isolation measures, wearing surgical face masks to the advent of vaccines. The rapid placing of vaccines on the market has caused resistance to vaccination in a large number of people, possibly due to insufficient information, misinformation or fear. Causing serious illnesses and the influx of a large number of patients, the COVID-19 pandemic has had a negative impact on the healthcare system in the form of utilization of healthcare resources. Healthcare workers are given the task of increased care and concern for the sick. For the purpose of researching the knowledge and attitudes of healthcare professionals about vaccination against the SARS-CoV-2 virus, as well as for the purpose of researching the attitude of healthcare professionals about the unvaccinated population against the mentioned disease, a questionnaire was created in which 132 respondents participated. The questionnaire provided an insight into how the majority of health professionals were involved in working with patients, how a large number of respondents believe that the vaccine is not necessary, and the majority of respondents are afraid of the risks of vaccines. Most of the surveyed health professionals do not consider the refusal of vaccination against the disease COVID-19 as a reason why patients should bear the costs of hospitalization themselves or should not worry about them. Also, the majority of respondents do not consider unvaccinated people as a population that did not contribute to the fight against the COVID-19 pandemic. Guided by the principles on which the health profession rests, health workers are obliged to take care of the patient in their work, regardless of their own choice of a certain medical procedure.