Human Papillomavirus (HPV) is currently the most common sexually transmitted infection with an unresolved etiology, posing a new challenge among sexually transmitted diseases. HPV is divided into two main groups: high-risk and low-risk. Although only a small percentage of infections progress and cause further complications, it is essential to take action on multiple levels to protect against it. Persistent high-risk genital HPV infection can lead to cervical cancer, a connection proven by the German scientist Harald zur Hausen. Therapeutic procedures cannot completely eliminate HPV because there are no specific antiviral drugs. Therefore, treatment focuses on removing visible changes to prevent disease progression and reduce the possibility of transmission. As there are currently no effective means of preventing HPV transmission, complete sexual abstinence is the only way to protect against infection. Risk factors for infection are related to sexual behavior, including early sexual initiation and having multiple sexual partners. HPV also causes cancer in men, and prophylactic vaccination is a mechanism for reducing HPV infection in men. Epidemiological data indicate that men are a viral reservoir, which is a phenomenon that must be addressed to reduce HPV infections at the population level. A study was conducted on a portion of the general population regarding participants' attitudes, practices, and knowledge about HPV through an online questionnaire using Google Forms in February and March 2022. The research aimed to assess the population's level of knowledge about HPV, examine their attitudes toward HPV, and investigate whether participants adhere to measures for protection and prevention of HPV infection. The study involved 323 anonymous participants. The questionnaire consisted of a total of 22 questions divided into 4 sections. One part of the survey examined the personal socio-demographic characteristics of the participants, while the other part explored participants' sexual practices and knowledge about HPV. The study was voluntary and anonymous. It included 237 women and 86 men, with the majority of participants in the age range of 18 to 30 years and mostly having a secondary level of education. The results indicate a lack of knowledge in the general population about HPV, particularly regarding transmission methods and preventive measures. To protect young people from potential HPV infection, the most effective measure is comprehensive awareness about the virus, its characteristics, and preventive measures. Raising the level of knowledge is achieved through educational programs, implementing sexual education in schools, and promoting responsible sexual behavior. Social media has a significant influence on young people and is a means of spreading information. Socio-demographic data, sexual habits, preventive screenings, and vaccination data are important factors related to HPV infection that should be continuously monitored and researched to invest efforts in ensuring and improving preventive measures against HPV. Developed countries need to raise awareness about the importance of vaccination, considering the percentage of those who do not get vaccinated, while developing countries need to allocate more resources to screening programs.