Toxoplasma gondii is a parasite found in almost all warm-blooded animals, birds, and humans. T. gondii is a protozoan that causes the parasitic disease toxoplasmosis. In humans, it appears in three forms, as congenital, transplacentally transmitted infection or acquired toxoplasmosis. With acquired toxoplasmosis, we have no pronounced symptoms or they can be very mild, but in immunocompromised people it can cause severe complications. Domestic cats and other animals from the feline family are the final carriers of the T. gondii parasite, and sexual reproduction of the parasite takes place in them. Other animals and man are only transitional carriers. For the purpose of creating this thesis, a research study entitled "Knowledge level, attitudes and perceptions of pregnant women and mothers about the parasite Toxoplasma gondii (T. gondii)" was conducted. The survey questionnaire contained 19 questions, 120 respondents participated, and it took about 5 to 10 minutes to solve it. The survey questionnaire was conducted from September 29 to October 11, 2023. The obtained data are indicators that the education of women during pregnancy and before pregnancy is not adequate and complete. Advancement is needed in primary health care itself, and there is a public health message for the need for more education about certain infections. Therefore, the level of knowledge of pregnant women and mothers about this parasite is crucial for the prevention of infection, and pregnant women should be informed and instructed about the ways of transmission, recognition of symptoms and prevention measures. Education and awareness of the risks can affect behavior change and adherence to preventive measures, that is why the role of the bachelor's degree in nursing is important here to prevent infection with this parasite by educating pregnant women, and the most important thing is to adhere to preventive measures, and it is one of the cheapest options. The attitudes and perceptions of pregnant women and mothers about this parasite play a significant role in protecting them and their families from toxoplasmosis. Based on the research conducted, we can conclude that a small number of subjects were informed and educated about toxoplasmosis, and a small number of subjects were tested for IgG and IgM antibodies against T. gondii to diagnose toxoplasmosis, which is worrying information. Public awareness should be raised about the harmfulness of infection with the parasite T. gondii during pregnancy and the level of knowledge about preventive measures should be raised, which is the cheapest and fastest solution for the entire population and would be beneficial in protecting children, mothers and families.