Even today, after more than thirty years since the discovery of the human immunodeficiency virus (HIV), controling the outbreak of the infection with this agent is one of the most important global public health prioritie. HIV infection is characterized by the extenuating transmission via sexual pathways, blood, transplantation of the infected organs, and transmission mother to child. Most infections occur after sexual exposure, homosexual or heterosexual transmission, which is why HIV infection is primarily considered a sexually transmitted disease. After HIV enters the host organism, it starts off several years of weakening of the person’s immune system with the development of numerous clinical events – from asymptomatic HIV infection to the last stage of HIV disease, which we call AIDS, accompanied by opportunistic infections and tumour formation. The specificity of this agent is the inability to tackle it with medical treatment, resulting in a large number of deaths. In addition to a sufficiently difficult life with HIV, diseased individuals are often targeted by stigmatising attitudes of the population and a high level of discrimination related to all aspect of their lives – including education, employment and health care. Stigmatisation does not bypass the sick even within the health care system, and such situation has a particularly negative effect on measures for prevention of infection and obtaining adequate health care which is crucial for achieving the optimal quality of life of these patients. Since nurses/technicians are health care professionals, the presence of stigma in these groups of health care professionals is detrimental because it significantly affects the quality of the provided medical care. Accordingly, for the purposes of this thesis, in the period between 6 and 13 April, 2020, an online questionnaire was carried out exploring stigmatization of patients with HIV/AIDS. The aim of the research was to determine the existence and frequency of stigmatising attitudes of nurses/technicians towards HIV positive persons and persons with AIDS. Also, this thesis tried to get an adequate insight into the participants’ experience and knowledge. A total of 757 participants participated in the survey, of which 94.3% were nurses and 5.7% were medical technicians. The results show than 81.2% of participans in this survey have a positive attitude towards a person living with HIV/AIDS, but the remaining 18.8% of participants (despite the progress of medicine and education) still have a large number of negative attitudes, suggesting that the stigma is still present towards this group of patients within our health care system and that this problem still necessitates effective strategies.