Pain is universal and subjective human experiance, it is concidered fifth vital sign and more and more importance is placed as a symptom. There are several kinds of pain: acute pain, pain caused by malignant disease and chronic non-maligant pain. The origin od pain consists of several stages; transduction, conduction, transmission, perception and perception of pain sensation. We divide pain according to various different criteria; according to cause (etiology) - into malignant and non-malignant pain, pathophysiological mechanisms -into neuropathic and nociceptive pain and the duration of pain - acute and chronic pain. Control of breakthroug pain is important. In treatment, a multidisciplinary approach is important. We use pharmacological therapy and non-pharmacological pain treatment. There are different methods of non-pharmacological treatment such as: change of position, relaxation, occupational therapy, hypnosis, acupuncture, massage, biofeedback and guided imagination. To assess pain, we use unidimensional and multidimensional pain assessment scales. Unidimensional scales in practice are used more often, while Multidimensional scales for pain assessment provide us with more information about pain than unidimensional ones. The aim of this research was to examine the level of knowledge and experience of students at the University of North about the assessment and treatment of pain. For the purposes of this work, a survey questionnaire was prepared, which was filled out by 47 participants. The data was analyzed and tabulated. It is evident from the results that the students have knowledge and experience about the assessment and treatment of pain with patients. They apply their knowledge in working with patients.