Healthcare associated infections develop during hospital treatment or after discharge from the hospital. They can appear individually or epidemically, and are determined by microbiological and laboratory testing. Improper hand hygiene of healthcare workers and failure to use disinfectants is considered the most common way of spreading Clostridioides difficile is an anaerobic sporogenous bacterium that has great resistance and the ability to survive in the hospital environment, even for several months. The mentioned bacterium, Clostridioides difficile, is the most common cause of diarrhea in healthcare institutions, and epidemics are more common in institutions for the elderly, who are also a high-risk population due to reduced gut microflora and already present comorbidities. The basis of epidemic prevention is the education of healthcare workers, the regulation of the use of antibiotics, and the proper hand hygiene of healthcare workers and (the hygiene of) the environment where patients stay. For the purposes of writing this final paper, an online questionnaire was conducted using a Google form. Respondents filled out the questionnaire anonymously and voluntarily, and a total of 102 respondents were interviewed. The questionnaire contained five questions that examined socio-demographic data and 21 questions that tested their knowledge about Clostridioides difficile infection in homes for the elderly and infirm. The goal was to examine the recognition of symptoms of infection, as well as knowledge and the ways of creating and spreading that knowledge and awareness. Respondents aged 20 to 60 and over took part in the research, of which the majority were in the 31 to 40 age range, 39.2% of them. A total of 65.7% were female respondents and 34.3% were male respondents. With regard to the level of education, the majority of respondents have a high school diploma, i.e. 58.8% of them. The majority of respondents, 44.1%, have up to five years of work experience in institutions for the elderly. Based on the knowledge test, it can be determined the respondents showed an appropriate level of knowledge about Clostridioides difficile infection, and that this knowledge is a factor in the recognition, treatment and the spread plan of important infections.