Intrahospital infections represent a significant public health problem in developed and developing countries. Reducing the prevalence of nosocomial infections is critical to providing safe and high-quality health care, even in resource-limited settings. Transmission of infection between patients by healthcare workers and irrational use of antibiotics have been identified as some of the most important risk factors that can be influenced. Nurses are the largest group in the healthcare system, and they work in close contact with patients. The goal of nurses is to provide quality healthcare, however, the problems faced by the healthcare system, such as the lack of healthcare professionals and economic problems, make their daily work, and thus the safety of patients, difficult. The research that was carried out for the purpose of creating this work is based on 131 respondents who work as nurses/technicians in the hospital system and in outpatient activities. The degree of adherence to certain guidelines and recommendations in daily work with patients was examined, as well as the statistical significance of certain variables with regard to the level of education, age, work experience and department of employment. Also, the respondents had the opportunity to state what, in their opinion, are the reasons for non-compliance with guidelines and recommendations. The obtained results confirm the set hypotheses. At the level of p<0.05, nurses/technicians with a completed secondary vocational education are significantly less likely to adhere to certain guidelines compared to respondents with a completed graduate/postgraduate professional nursing study and a postgraduate doctoral study. With regard to years of life, and at the p<0.05 level, respondents aged 20 to 39 adhere to the guidelines statistically significantly less than respondents older than 40. According to the length of work experience, respondents with 6 to 10 years of work experience and those with more than 20 years of experience adhere best to the guidelines. The largest number of respondents cited a lack of time due to the increased volume of work, a lack and staff negligence as the reason for not following guidelines and recommendations.