Introduction: The research was conducted on patients of the Department of Urology who underwent diagnostic urethrocystoscopy in order to evaluate the impact of preparation for the procedure on pain. The research lasted from March 1, 2023. until 01.05.2023. Objective: To prospectively assess the level of pain self-reported by patients after diagnostic urethrocystoscopy. Methods: Patients were given informed consent to participate in the study. During the research, basic epidemiological data on gender, age, previous urethrocystoscopies and the quality of the information provided about the procedure were collected. Pain was rated on a Likert visual analog scale (VAS) from 1-10. Data were collected during March and April 2023. Results: The research was conducted on 134 patients, of whom 77 were men and 57 were women. The median age of the respondents is 64, with the largest number of patients aged 61 to 70. The median pain score in the entire patient sample was 2. Men had a significantly higher pain score than women (median 3 vs. median 2). Among men who underwent urethrocystoscopy for the first time, a higher pain score was recorded compared to those who had already undergone it, while there were no significant differences among women. Conclusions: Gender and age have a major impact on the patient experience during urethrocystoscopy. Information given before the procedure can negatively affect the perception of anxiety in patients, so it is recommended to improve the way information is provided. The research did not show an association of uretocystoscopy with high levels of pain.