Acute ischemic stroke (AIMU) is an emergency medical condition that occurs when a blood vessel in the brain is blocked by a thrombus or embolus, which cuts off blood flow to a certain part of the brain. AIMU is a disease of sudden and unpredictable onset, which occurs regardless of age or gender. The resulting symptoms are diverse and depend on the intensity and location of AIMU. Since AIMU is an emergency, it is extremely important to react quickly. One of the main therapeutic options for the treatment of AIMU is systemic thrombolytic therapy. The goal of thrombolysis is to dissolve the thrombus or embolus in order to save the penumbra and restore circulation to the affected ischemic area. The goal of this study was to examine the usefulness of treatment with systemic thrombolytic therapy. The analyzed data were collected from the Hospital Information System (BIS) of the Neurology Department of General Hospital Varaždin in the period from 2019. - 2023. The study analyzed 123 patients hospitalized for AIMU, who were treated with systemic thrombolytic therapy. The research examined how much thrombolysis was performed in relation to all hospitalized AIMUs, at what age or gender the occurrence of AIMUs is the highest, at what time of day AIMUs occurred, the incidence and type of symptoms, the time of the patient's arrival at the hospital, the time of examination and treatment of the patient before thrombolysis, and the time window in which the patients received systemic thrombolytic therapy. The recovery of patients with the time window after systemic thrombolytic therapy was also examined and compared, and the place of discharge of patients was examined. Systemic thrombolytic therapy treated 7.04% of patients, out of all hospitalized AIMUs. More men than women underwent thrombolysis, and the average age of the patients was 66.5 years. AIMU most often occurred in the morning, while the onset of symptoms was most often in the form of facioparesis, hemiparesis or ataxia and dysphasia or dysarthria. Patients arrived at the hospital in an average of 76 minutes, the average DNT was 80 minutes and the average time window was 166 minutes. Patients who received thrombolysis in a shorter time window showed better recovery results, and in half of the patients the treatment ended with discharge home. Research has shown that treatment with systemic thrombolytic therapy is effective, especially if it is applied in as short a time window as possible. In the overall process of treatment of AIMU with systemic thrombolysis, an important role is played by masters of nursing who are responsible for carrying out nursing interventions, providing support, supervision and monitoring the patient's condition, and predicting the course of thrombolysis. They also have a role in organizing, delegating and verifying the correctness of nursing activities. Masters of Nursing can implement public health campaigns and educational programs for the general population aimed at recognizing the symptoms of MU and emphasize the importance of seeking emergency care quickly.