Healthcare-associated infections (HAIs), also known as hospital-acquired or nosocomial infections, are infections acquired by patients during the provision of healthcare that were not present or incubating upon admission to a healthcare facility. These infections can occur in hospitals, clinics, clinics, or long-term care facilities, such as nursing homes. These include a wide range of infections, such as urinary tract infections, surgical site infections, respiratory infections, and bloodstream infections. HAIs can have significant consequences, including increased morbidity and mortality among patients, prolonged hospital stays, and significant additional costs to healthcare systems. The causative agents of HAIs often include bacteria, viruses, and fungi, with some of the most common pathogens standing out for their resistance to standard treatments, such as methicillinresistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococci (VRE). HAI prevention and control require comprehensive strategies that include strict adherence to infection control protocols, proper hand hygiene, use of personal protective equipment (PPE), adequate disinfection and sterilization of equipment, and education of health care personnel and patients about the importance of preventive measures. Also, the rational use of antibiotics is essential to reduce the occurrence of antimicrobial resistance, which is an increasingly pronounced problem in the control of HAIs. Highly educated nurses play a key role in this issue, as they are often on the front line of providing care and infection prevention through the implementation and supervision of hygiene and sterilization practices.