Industry 4.0 as part of the fourth industrial revolution marks the transformation of the business by integrating information and communication technologies to increase the productivity and efficiency of organizations. The application of Industry 4.0 is the main challenge for organizations and requires the collaboration of business entities, politics, and the academic community. The process of implementation is affected by several internal and external challenges that the organization has to deal with. Therefore, it was necessary to define the influencing factors that will enable organizations to better understand and provide guidelines for implementation. The paper defines the key factors that affect the success of the Industry 4.0 system in the organization. The key factors are defined by searching and analyzing papers on the topic of implementation and Industry 4.0. At the end of the paper, the advantages and disadvantages of Industry 4.0 are listed, and a practical overview of the application of 4.0 technologies in the food industry is shown.