This final paper deals with the analysis of the attitudes and level of knowledge of students of health and non-health professions about vaccination against the human papillomavirus (HPV). HPV is considered the most common sexually transmitted infection, and causes various types of cancer, including cancer of the cervix, penis, vagina, and oropharyngeal regions. HPV Risk include early onset of sexual activity, greater number of sexual partners, unprotected sex, low level of education, smoking, and reduced immunity. Treatment of HPV infections includes various methods, depending on the nature of the lesions. For benign lesions, such as genital warts, treatment may include cryotherapy, electrocautery, and chemical methods. HPV prevention includes vaccination as the most effective form of protection against the most dangerous strains of the virus. Vaccination against HPV has been available in the Republic of Croatia since 2015, free of charge for primary and secondary school students and for all persons up to 25 years of age. In addition to vaccination, regular examinations and testing, such as the PAPA test, are recommended in order to timely detect possible changes caused by HPV infection. The objective of the research was to determine how informed the students are about HPV, what their attitudes are towards vaccination, and the actual vaccination rate among them. The research was conducted through a questionnaire on a sample of 261 students, divided into two groups: students of health and non-health professions. The results showed that students of health professions have better information and a higher level of knowledge about HPV and vaccination compared to their colleagues from non-health professions. However, regardless of the relatively high level of information, most students show a negative attitude towards vaccination - only a small part of them have been vaccinated, and many unvaccinated students do not plan to do so in the future.