Women in entrepreneurship face numerous challenges stemming from cultural, social, and economic circumstances. Despite the increasing number of women in the business world, barriers that hinder their success still persist. This paper analyzes these obstacles, including discrimination, social stereotypes, difficulties in accessing finance, and balancing personal and professional responsibilities, with a particular focus on institutional support and cultural norms. The research is based on the analysis of women entrepreneurs in Croatia and a quantitative study involving 190 respondents. The main objective of the research was to examine the specific obstacles and challenges women face in the business world, as well to explore political and institutional measures that could help overcome them. The research questions address the social and cultural norms that shape business opportunities for women, the connection between family responsibilities and their business activities, and the existing strategies for supporting female entrepreneurship. The research results confirm that women entrepreneurs face greater challenges in business than their male counterparts, especially due to cultural norms, discrimination, and stereotypes. Furthermore, balancing business life and family responsibilities represents a significant barrier, while political and institutional measures, such as increased financial support and flexible working conditions, can significantly reduce those obstacles. Support for female entrepreneurship in the form of educational programs, mentoring networks, and financial assistance is also considered important for reducing barriers. The study shows that women entrepreneurs are mostly supported by their families, but institutional and legal support is also crucial for overcoming challenges. This paper emphasizes the importance of further initiatives and strategies to reduce the obstacles that women entrepreneurs encounter and enable them to fully utilize their potential. It is recommended to continue developing clear and effective support measures that can help create balance in the business sector and promote gender equality.